Qpcr standard deviation

As all of you probably know, there are two methods for relative quantification of gene expression from qPCR data, the double delta Ct (ΔΔCt) method and the Pffafl method.

Chances are you’ve probably stuck with the ΔΔCt method, but you should be prepared in case you face primer sets of different amplification efficiencies.

Both methods require the use of a housekeeping gene to control for differences in sample quantity, and both report the results as a fold change in the expression of the target gene in test samples, relative to control samples.

But what is the difference between them?

In this article, we are going to explain both of these methods and highlight when to use each.

What is Relative Quantification?

Relative quantification in qPCR is where you measure gene expression levels by comparing the levels of expression of your gene of interest against the levels of expression of an internal control gene. 

This offers a fairly simple way to determine if the expression of your gene of inter

rq in pcr

rq calculation